Pirfenidone suppresses the abnormal activation of human Müller cells after platelet-derived growth factor-BB stimulation
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Hua Zhong. Department of Ophthalmology, the First Affiliated Hospital of Kunming Medical University, Kunming 650031, Yunnan Province, China. zhoculist@163.com

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    Abstract:

    AIM: To determine the effect of pirfenidone on the activated human Müller cells by platelet-derived growth factor-BB (PDGF-BB). METHODS: The primary human Müller cells were separated from retinal tissues and established the pathogenic model by stimulated with PDGF-BB. The Müller cells behaviour of normal group and the model group was measured by MTT assay, Trypan blue assay, cell migration assay, and collagen contraction assay. The expression of transforming growth factor (TGF)-β1, -β2, and pigment epithelium-derived factor (PEDF) was estimated with real-time polymerase chain reaction (PCR), Western blot and immunofluorescence analyses. RESULTS: A pathogenic/proliferative model of Müller cells was established by stimulating normal cultured Müller cells with 10 ng/mL PDGF-BB for 48h. After treated with 0.2 and 0.3 mg/mL pirfenidone, the proliferation, migration and collagen contraction was statistically significantly depressed in the model group compared with the normal groups. The expression levels of TGF-β1 and TGF-β2 were significantly down-regulated, while the PEDF expression was significantly up-regulated after treated with 0.2 and 0.3 mg/mL pirfenidone in the model group. CONCLUSION: Pirfenidone effectively suppress the proliferation, migration and collagen contraction of the human Müller cells stimulated with PDGF-BB through down- regulation of TGF-β1/TGF-β2 and up-regulation of PEDF.

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Yi-Jin Tao, Qin Chen, Li Wang, et al. Pirfenidone suppresses the abnormal activation of human Müller cells after platelet-derived growth factor-BB stimulation. Int J Ophthalmol, 2019,12(7):1075-1082

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Publication History
  • Received:January 03,2019
  • Revised:March 11,2019
  • Adopted:
  • Online: June 11,2019
  • Published: