Citation:Huan Y,Peng XD,Lin J,Zhang YX,Zhan L,Gao H,Zhao GQ.Anti-inflammatory effects of astaxanthin against fungal keratitis.Int J Ophthalmol 2020;13(11):1681-1688,doi:10.18240/ijo.2020.11.01
Anti-inflammatory effects of astaxanthin against fungal keratitis
Received:January 12, 2020  Revised:August 14, 2020
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DOI:10.18240/ijo.2020.11.01
Key Words:astaxanthin  fungal keratitis  Aspergillus fumigatus  anti-inflammation  neutrophil
Fund Project:Supported by the National Natural Science Foundation of China (No.81870632); Youth Project of Natural Science Foundation of Shandong Province (No.ZR2019BH004).
                    
AuthorInstitution
Yu Huan Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao , Shandong Province, China
Xu-Dong Peng Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao , Shandong Province, China
Jing Lin Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao , Shandong Province, China
Ying-Xue Zhang Department of Biochemistry, Microbiology and Immunology, Wayne State University School of Medicine, MI 48201, USA
Lu Zhan Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao , Shandong Province, China
Han Gao Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao , Shandong Province, China
Gui-Qiu Zhao Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao , Shandong Province, China
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Abstract:
      AIM: To characterize effect of astaxanthin (ASX) in Aspergillus fumigatus (A. fumigatus) induced keratitis in mouse model.

    METHODS: In vivo, fungal keratitis mouse model was established in C57BL/6 mice using A. fumigatus, followed by ASX or dimethyl sulfoxide (DMSO) treatment. Clinical responses were evaluated by clinical score and myeloperoxidase (MPO) assay. Inflammatory cytokines were assessed by reverse-transcription polymerase chain reaction (RT-PCR), Western blot, immunofluorescence, and enzyme-linked immuno sorbent assay (ELISA).

    RESULTS: In animal model, ASX improved corneal transparency and clinical response, suppressed the expression of inflammatory cytokine like IL-1β, TNF-α, and HMGB-1. Neutrophil levels have been shown to decrease in ASX-treated cornea by immunofluorescence and MPO. TLR2 and TLR4 levels were lower in ASX-treated group than DMSO-treated.

    CONCLUSION: ASX can suppress inflammatory response and reduce inflammatory cytokine production in mice model with A. fumigatus keratitis.

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