Expression and role of specificity protein 1 and collagen I in recurrent pterygial tissues
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Bo Jiang. Department of Ophthalmology, Anhui No.2 Provincial People’s Hospital, No.1868 Dangshan Road, Hefei 230041, Anhui Province, China. 625505145@qq.com

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Supported by the Key Program of Natural Science Research of Anhui Provincial Education Department (No.KJ2019A1097); Science Foundation of Anhui Provincial Health Bureau (No.2018SEYL025); Natural Science Research of Anhui Provincial Education Department (No.12925KJ2018B11).

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    Abstract:

    AIM: To investigate the expression profiles of the transcription factor specificity protein 1 (Sp1) and collagen I in recurrent pterygial tissues. What is more, to compare the changes of Sp1 and collagen I among primary pterygial tissue, recurrent pterygial tissue and conjunctival tissue. METHODS: In the prospective study, we collected the pterygial tissues of 40 patients who underwent resection of primary pterygial tissue and recurrent pterygial tissue, and the conjunctival tissues of 10 patients with enucleation due to trauma. The relative expression levels of Sp1 and collagen I were analyzed by reverse transcription quantitative-polymerase chain reaction and Western blot. Paired t-test was performed to compare the Sp1 and collagen I of recurrent pterygial tissues, as well as the primary pterygial tissues and conjunctival tissues. In further, Pearson’s hypothesis testing of correlation coefficients was used to compare the correlations of Sp1 and Collagen I. RESULTS: The content of Sp1 and collagen I mRNA and protein was significantly greater in recurrent pterygial tissue than that was in primary and conjunctival tissue (P<0.05). There was a positive correlation between the mRNA and protein levels of Sp1 and collagen I in recurrent pterygial tissues (protein: r=0.913, P<0.05; mRNA: r=0.945, P<0.05). CONCLUSION: Sp1 and collagen I are expressed in normal conjunctival, primary, and recurrent pterygial tissues, but expression is significantly greater in the latter. Sp1 and collagen I may be involved in the regulation of the development of recurrent pterygium.

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Chun-Sheng Shi, Yue Wu, Na Shu, et al. Expression and role of specificity protein 1 and collagen I in recurrent pterygial tissues. Int J Ophthalmol, 2021,14(2):223-227

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Publication History
  • Received:May 05,2020
  • Revised:August 11,2020
  • Adopted:
  • Online: December 24,2020
  • Published: