AIM: To analyse the changes in magnitude and orientation of astigmatism after suture removal (SR) in keratoplasty eyes as measured by refraction, tomography, and aberrometry. METHODS: Twenty-six eyes of 25 patients after optical keratoplasty requiring SR to reduce the astigmatism during the follow-up period were prospectively included. Eyes with poor quality topography scans or if there were no sutures in the steepest semi meridian were excluded. Corrected distance visual acuity (CDVA), manifest refraction, corneal tomography and aberrometry were performed on all patients before and after SR. RESULTS: The mean age of the patients was 40.8±14.4y. Penetrating keratoplasty was performed in 23 eyes (89%) and deep anterior lamellar keratoplasty was done in 3 eyes (11%). There was a statistically significant reduction in the magnitude of refractive, tomographic and aberrometry astigmatism after SR (P<0.001) at 2h after suture removal. The mean net reduction of the astigmatism was greater as measured by corneal tomography compared to refractive astigmatism (P<0.05). There was no statistically significant change in refractive astigmatism between 2h and 2mo after SR (P=0.55). Vector calculations demonstrated a greater amount of undercorrection in the tomography group and the rotational error was more towards counterclockwise direction. Mean monocular logMAR CDVA improved from 0.57 D to 0.49 D after SR (P=0.002). CONCLUSION: The net reduction in the magnitude of astigmatism after SR is greater in the tomography and aberrometry groups. With one episode of SR, there is no difference in the aberration profile.