Leptin activates the JAK/STAT pathway to promote angiogenesis in RF/6A cells in vitro
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Jun-Hui Du. Department of Ophthalmology, Xi’an Ninth Hospital Affiliated to Medical College of Xi’an Jiaotong University, Xi’an 710054, Shaanxi Province, China. djh79918@163.com

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Supported by the Matching Funds of the National Natural Science Foundation of China (No.XYFYPT-2020-01); the Natural Science Foundation of Shaanxi Province (No.2020JM-685; No.2021JM-547); the Fundamental Research Funds for the Central Universities (No.1191329116); the Foundation of Xi’an Health Committee (No.2020MS07).

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    Abstract:

    AIM: To investigate the effect of leptin on the angiogenesis of RF/6A cells (monkey retinal choroidal endothelial cells) in vitro and test the cellular signaling in the mechanism. METHODS: RF/6A cells were cultured in vitro and randomly divided into four groups: normal control, with leptin at 50, 100, 200 ng/mL for cell counting kit-8 (CCK8). RF/6A cell proliferation and migration were examined by Transwell assays, while RF/6A cell tube formation by Matrigel assay. JAK2, p-JAK2, STAT3, and p-STAT3 protein expression was measured by Western blotting. Cells were then divided into the following treatment groups: control, 100 ng/mL leptin and AG-490 (100 ng/mL leptin+10 μmol/L AG-490) for examinations of RF/6A cellular behaviour again. Analysis of differences was carried out using one-way ANOVA and least significant difference (LSD). RESULTS: RF/6A cell proliferation, migration and cell tube formation were promoted significantly by leptin in a dose-dependent manner (P<0.05). Western blotting showed that leptin up-regulated p-JAK2 and p-STAT3 expression levels. Treatment with the JAK/STAT pathway inhibitor, AG-490, decreased leptin-induced p-JAK2 and p-STAT3 expression, and inhibited cell proliferation, migration and cell tube formation induced by leptin (P<0.05). CONCLUSION: Leptin can promote RF/6A cell angiogenesis in vitro via activation of the JAK2/STAT3 signaling pathway.

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Le Zhang, Rong Li, Bing-Hui Wu, et al. Leptin activates the JAK/STAT pathway to promote angiogenesis in RF/6A cells in vitro. Int J Ophthalmol, 2022,15(4):554-559

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Publication History
  • Received:October 14,2021
  • Revised:January 26,2022
  • Adopted:
  • Online: March 29,2022
  • Published: