Protective limb remote ischemic post-conditioning against high-intraocular-pressure-induced retinal injury in mice
Author:
Corresponding Author:

Jun Wang. Henan International Joint Laboratory for Nuclear Protein Regulation, Henan University, Kaifeng 475001, Henan Province, China. whb21c@vip.henu.edu.cn

Affiliation:

Clc Number:

Fund Project:

Supported by the National Natural Science Foundation of China (No.31300884; No.81803573).

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    AIM: To determine whether limb remote ischemic post-conditioning (LRIC) protects against high-intraocular-pressure (IOP)-induced retinal injury, and to identify underlying molecular mechanisms. METHODS: In mice, IOP was increased to 110 mm Hg for 50min and LRIC applied to the unilateral leg for three occlusion cycles (5min/release). Three animal groups (control, high IOP, and high IOP+LRIC) were arranged in this study. Plasma was collected from LRIC treated mice. Retinal histology, oxidative stress were determined by histological section staining and chemical kit. C/EBP homologous protein (CHOP), and Iba-1 parameters were evaluated by immunofluorescent staining and Western blot. RESULTS: The data showed that LRIC treatment alleviated the retinal histological disorganization and ganglion cell loss induced by high IOP. The CHOP, Iba-1 expression and oxidative stress marker also were inhibited by LRIC treatment. To further explore underlying mechanisms, plasma from LRIC treated animals was intravenously transfused into high-IOP animals. The results showed plasma injection decreased caspase 9 expression and DHE staining signals compared with that in high IOP retinas. CONCLUSION: These data suggest that LRIC treatments exert retinal protective effects against high-IOP injury. Endogenous humoral factors release into the circulation by LRIC may contribute to homeostatic protection by reducing monocyte infiltration and/or microglia activation.

    Reference
    Related
    Cited by
Get Citation

Qi-Jiang Zhu, Lei Zhang, Shuang-Yu Lyu, et al. Protective limb remote ischemic post-conditioning against high-intraocular-pressure-induced retinal injury in mice. Int J Ophthalmol, 2022,15(4):560-567

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
Publication History
  • Received:June 30,2021
  • Revised:November 02,2021
  • Adopted:
  • Online: March 29,2022
  • Published: