CD3ε of a pan T cell marker involved in mouse Aspergillus fumigatus keratitis
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Cheng-Ye Che. Department of Ophthalmology, the Affiliated Hospital of Qingdao University, No.369 of Shanghai Road, Pingdu District, Qingdao 266000, Shandong Province, China. chechengye@126.com

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Supported by the National Natural Science Foundation of China (No.82171019); the Natural Science Foundation of Shandong Province (No.ZR2021MH368); Traditional Chinese Medicine Research Project of Qingdao (No.2020-zyy055); Shandong Qingdao Outstanding Health Professional Development Fund.

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    Abstract:

    AIM: To explore whether CD3ε is involved in the adaptive immunity of Aspergillus fumigatus (A. fumigatus) keratitis in mice and the role of innate and adaptive immunity in it. METHODS: Mice models of A. fumigatus keratitis were established by intra-stromal injection and corneal epithelial scratching. Subconjunctival injections of natamycin, wedelolactone, LOX-1 inhibitor (poly I) or Dectin-1 inhibitor (laminarin) were used to treat mice with A. fumigatus keratitis. Mice were pretreated by intraperitoneal injection of anti-mouse CD3ε. We observed the corneal infection of mice under the slit lamp microscope and made a clinical score. The protein expression of CD3ε and interleukin-10 (IL-10) was determined by Western blotting. RESULTS: With the disease progresses, the degree of corneal opacity and edema augmented. In the intra-stromal injection models, CD3ε protein expression began to increase significantly on the 2nd day. However, in the scraping epithelial method models, CD3ε only began to increase on the 3rd day. After natamycin treatment, the degree of corneal inflammation in mice was significantly attenuated on the 3rd day. After wedelolactone treatment, the severity of keratitis worsened. And the amount of CD3ε protein was also reduced, compared with the control group. By inhibiting LOX-1 and Dectin-1, there was no significant difference in CD3ε production compared with the control group. After inhibiting CD3ε, corneal ulcer area and clinical score increased, and IL-10 expression was downregulated. CONCLUSION: As a pan T cell marker, CD3ε participate in the adaptive immunity of A. fumigatus keratitis in mice. In our mice models, the corneas will enter the adaptive immune stage faster. By regulating IL-10, CD3ε exerts anti-inflammatory and repairs effects in the adaptive immune stage.

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Wen-Hao Shi, Li-Mei Wang, Hai-Jing Yan, et al. CD3ε of a pan T cell marker involved in mouse Aspergillus fumigatus keratitis. Int J Ophthalmol, 2024,17(4):616-624

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Publication History
  • Received:November 05,2023
  • Revised:January 12,2024
  • Adopted:
  • Online: March 26,2024
  • Published: