AIM: To evaluate retinal hemoglobin oxygen saturation in myopic eyes by scanning laser ophthalmoscope (SLO) and to assess its correlations with different severity of myopia. METHODS: Sixty-one eyes from 61 patients were included and subdivided into three groups according to their refractive errors: high myopia group [20 eyes, spherical equivalent (SE)≤-6 D]; low and moderate myopia (22 eyes, -6.00.05). CONCLUSION: SO2A and SO2V increased in more myopic eye based on SLO measurements. Further studies are warranted to investigate the changes of retinal hemoglobin oxygen saturation in myopia with different methods.