Inhibition of RhoA/Rho-kinase pathway suppresses the expression of extracellular matrix induced by CTGF or TGF-β in ARPE-19

Jing Zhu, Duy Nguyen, Hong Ouyang, Xiao-Hui Zhang, Xiao-Ming Chen, Kang Zhang

Int J Ophthalmol 2013;6(1):8-14. DOI: 10.3980/j.issn.2222-3959.2013.01.02

We ourselves recently reviewed our article published in Int J Ophthalmol entitled “Inhibition of RhoA/Rho-kinase pathway suppresses the expression of extracellular matrix induced by CTGF or TGF-β in ARPE-19” and would like to submit a correction for the following figures.

Figure 3 Immunofluorescent localization of fluorescently-labeled phalloidin F-actin staining in ARPE-19 cells. The nucleus was counter-stained with DAPI (blue), as determined by fluorescence microscopy  A: Vehicle-treated control cells remained tightly attached to each other; B: ARPE-19 cells were incubated in the presence of ROCK inhibitor Y27632 (10 ng/mL); C: ARPE-19 cells were incubated with TGF-β (10 ng/mL) for 48h; D: Y27362 pretreatment inhibited the morphological changes in ARPE-19 cells as induced by TGF-β; E: ARPE-19 cells were incubated with CTGF (10 ng/mL) for 48h; F: Serum-starved ARPE-19 cells were pretreated with Y27632 and subsequently treated with CTGF (10 ng/mL). Magnification, ×200. Bar, 50 μm.

Figure 4 Immunofluorescence staining for fibronectin laminin MMP2 and type I collagen in ARPE-19 cells treated by TGF-β, CTGF or CTGF ROCK inhibitor Vehicle-treated control cells show the expressing of type I collagen (A) and MMP2 (K). The expression of fibronectin (F) and laminin (P) were not observed in Vehicle-treated control ARPE-19 cells. The expression of type I collagen (B, C), fibronectin (G, H), MMP-2 (L, M) increased when cells were cultured in both TGF-β and CTGF. The protein level of laminin in CTGF (R) was higher than in TGF-β (Q). The expression of type I collagen (E), fibronectin (J), and MMP-2 (O) decreased but not laminin (T) when ARPE-19 cells were pretreated with Y27632 and then incubated in the presence of CTGF. Immunofluorescence staining with DAPI nuclear staining: Magnification, ×400. Bar, 100 μm.

We would like to emphasize that these corrections do not affect the conclusions of our study. We sincerely apologize for any inconvenience caused by our oversight and express our gratitude for your attention to this matter.

Kang Zhang

On behalf of all authors

Jing Zhu, Duy Nguyen, Hong Ouyang, Xiao-Hui Zhang, Xiao-Ming Chen, Kang Zhang

November 16, 2024