Magnetic resonance imaging(MRI) has been widely used in the optic nerve change of anatomy and function in glaucoma. MRI is a new and stable technology in assessing the injury of optic nerve.Now there are some kinds of method used widely:diffusion tensor imaging (DTI); functional magnetic resonance imaging(fMRI);manganese enhanced magnetic resonance imaging(MeMRI)and proton magnetic resonance spectroscopy (MRS). This artical reviewed about the applicatin of MRI in glaucoma.