AIM:To summarise the nursing experience of retro-grade catheterization in 48 cases with chronic dacryo-cystitis diseases.METHODS:All the 48 cases were carried out the operation of retrograde catheterization. During the whole process,they received meticulous care. RESULTS:All the lacrimal passages of 48 cases became patency after the operation. Two cases’ silicone tube slipped off three months later,however,their lacrimal passages were still patency. Three cases’ lacrimal passages became restenosis two years later,two cases became recanalization through the same operation.CONCLUSION:Retrograde catheterization is an effective treatment for chronic dacryocystitis diseases,and meticulous care is an important guarantee for this operation.