角膜移植后角膜新生淋巴管与新生血管间的关联(英文)
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R779.65

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中国国家自然科学基金资助项目(No.30700927);中国广东省科技计划资助项目(No.93068)


Corneal lymphangiogenesis correlates closely with hemangiogenesis after keratoplasty
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National Natural Science Fund of China(No.30700927);Guangdong Technology Program Foundation,China(No.93068)~~

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    摘要:

    目的:研究角膜移植后角膜新生淋巴管与新生血管间关联。方法:人角膜取自行二次角膜移植的19名患者。5核苷酸酶-碱性磷酸酶(5-nase-Alkaline phosphatase,5-NA-ALP)双重酶组织化学染色及淋巴内皮细胞受体(lymphatic vessel endothelial receptor,LYVE-1)、内皮细胞黏附因子-1(platelet endothelial cell adhesion modecule-1,PECAM-1)双重免疫组化法标记角膜中的新生血管和淋巴管,并进行淋巴管计数(lymphatic vessels counting,LVC)和血管计数(blood vessels counting,BVC),比较BVC与LVC之间的关联。结果:角膜中存在角膜新生血管12例(63%),存在角膜新生淋巴管5例(26%)。角膜新生淋巴管仅出现在血管化角膜中。角膜移植后BVC与LVC间呈显著性正相关(r=0.725;P<0.01)。结论:人角膜移植后角膜新生淋巴管与新生血管之间存在密切关联。

    Abstract:

    ·AIM:To examine the relationship between corneal lymphangiogenesis and hemangiogenesis after keratoplasty.·METHODS:Nineteen human corneas were obtained from 19 patients undergoing a second corneal transplantation in Zhongshan Ophthalmic Center in 2005.Blood and lymphatic vessels in human transplanted corneas were identified by lymphatic vessel endothelial receptor(LYVE-1)and platelet endothelial cell adhesion modecule-1(PECAM-1)immunohistochemistry,and double enzyme-histochemistry;then the association of corneal blood vessel counting(BVC)with lymphatic vessel counting(LVC)was examined.·RESULTS:Corneal hemangiogenesis was present in 12 cases(63%),and lymphangiogenesis occurred in 5 cases(26%)of human transplanted corneas.In addition,corneal lymphangiogenesis was only present in vascularized corneas.LVC was strongly and positively correlated with BVC(r=0.725;P<0.01).·CONCLUSION:Corneal lymphangiogenesis develops after keratoplasty,and strongly associates with hemangiogenesis.·

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凌士奇,柳灿,黎韦华,等.角膜移植后角膜新生淋巴管与新生血管间的关联(英文).国际眼科杂志, 2010,10(3):405-408.

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