·AIM:To study the protective effect of Ginaton and fleabane on retinal ganglion cells in rabbit chronic high intraocular pressure model.·METHODS:Chronic high intraocular pressure was created by injecting α-chymotrypsin(0.2mL)(2667.2μkat)into rabbit posterior chamber.Thirty-two rabbits of successful model were divided randomly into four groups,with 8 rabbits in each group,and then treated with different therapies as follows:intragastric administration with Ginaton 150mg/kg,once a day(Ginaton group);intragastric administration with fleabane 150mg/kg,once a day(fleabane group);intragastric administration with Ginaton and fleabane(combination therapy group)and control group.After 4 weeks of treatment,all groups were checked retinal ganglion cells apoptosis,normal ganglion cells counting,pathological changes of retinal layers with light microscopy.·RESULTS:Positive cells could be found in every group,the number of normal retinal ganglion cells was the largest in combination therapy group(889.00±40/mm2),the least in control group(581±24/mm2),P<0.01.In light microscopy,the worst arrangement of retinal layers was in control group,and the best was in combination therapy group.·CONCLUSION:Ginaton and fleabane could prevent high intraocular pressure caused apoptosis of retinal ganglion cells.·