目的:探讨HIF-1α和VEGF在早产儿视网膜病变鼠模型中的表达及意义。方法:建立氧诱导的早产儿视网膜病变新生鼠模型。Western-blot检测视网膜HIF-1α的表达,并以计算机图像分析仪进行分析;ELISA方法检测视网膜VEGF的表达。结果:与正常对照组比较,视网膜病变组在鼠出氧箱后5,10,15d视网膜HIF-1α表达逐渐增加(P<0.01),呈时间依赖关系。视网膜VEGF的表达分别为381.5±3.1,431.8±1.3和443.9±1.2ng/L,与对照组比较,均有显著增加(P<0.01)。结论:氧诱导视网膜病变中的HIF-1α和VEGF上调。

AIM: To explore the expression of hypoxia-inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) in an animal model of oxygen-induced retino-pathy of prematurity(ROP).·METHODS: An animal model of oxygen-induced ROP was established,Western-blot analysis was performed to detect the expression of HIF-1α in retinae ,and the picture was analyzed by computer image analysis meter,ELISA was performed to detect the expression of VEGF in retinae.·RESULTS: Compared with normal control group, the expression of HIF-1α gradually increased in retinae in the fifth day,the tenth day,and the fifteenth day in hypoxia condition in the oxygen-induced rats(P<0.01), in an dose-dependent manner. The expression of VEGF was respectively 381.5±3.1,431.8±1.3 and 443.9±1.2ng/L in retinae in the fifth day,the tenth day,and the fifteenth day in hypoxia condition in the oxygen-induced rats. Com-pared with normal control group, the expression of VEGF in all experimented groups was significantly increased(P<0.01).·CONCLUSION: The expression of HIF-1α and VEGF is up-regulated in the oxygen-induced ROP.

R774.1

中国广东省深圳市科技计划资助项目(No.200802060)~~