目的:在具有家族史的高度近视儿童中进行TIGR基因突变筛查。方法:对高度近视儿童中TIGR基因第三外显子进行毛细血管电泳测序检测,并对结果进行序列分析。结果:在近视组和对照组中均未发现TIGR基因突变。结论:未能发现TIGR基因突变与高度近视相关证据。

AIM:To screen TIGR/myocilin gene(MYOC) mutation in high myopic Chinese children with family history.METHODS:Gene sequencing was performed in exon 3 of the TIGR gene in high myopic Chinese Children.The coding sequence of TIGR exon 3 was screened by capillary electrophoresis sequencing.The sequence alterations were analyzed by bioinformatics.RESULTS:TIGR gene mutation was not found in high myopic patients and normal controls group.CONCLUSION:No identified gene mutation is found in TIGR gene in high myopic Chinese children.

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中国湖南省教育厅资助项目（No.07C155）