AIM:To investigate the effect of oligofectamine mediated platelet-derived growth factor-α(PDGF-α) receptor antisense oligodeoxyribonucleotide(ASODN) transfection on retinal pigment epithelium(RPE) proliferation and apoptosis in vitro.METHODS:After PDGF-α receptor ASODN was transfected into RPE with lipofectamineTM2000 at different concentrations,the proliferation of RPE was detected by MTT.Hoechst staining was used to display the apoptosis form of RPE.Flow cytometry method was applied to detect the change of cell cycle and apoptosis index.RESULTS:Compared with control group,PDGF-α receptor ASODN group could effectively inhibit the proliferation of RPE;the percentage of G0/G1 phase cells was enhanced and there was significance in increasing apoptosis cells(P<0.05).CONCLUSION:PDGF-α receptor ASODN could induce G0/G1arrest,inhibit cell proliferation,accelerate apoptosis in RPE cells.