AIM:To analyze the clinical application value of photopic negative response(PhNR)in amblyopia by comparing the normal adult and amblyopia adult of PhNR.METHODS:PhNR was recorded and analyzed from 30 normal adult eyes and 30 amblyopia adult eyes,using red flashes on a blue background and white flashes on a white background respectively.RESULTS:Amplitudes of PhNR in amblyopia were lower than those of the normal group by red flashes on a blue background and white flashes on a white background,there were statistically significant differences between them.Latencies of PhNR in amblyopia were similar to normal group,there were no statistically significant differences between them.Amplitudes of PhNR by using white flashes on a white background were lower than those by using red flashes on a blue background with statistically significant differences,latencies of PhNR by white flashes on a white background were similar to those by red flashes on a blue background,there were no statistically significant differences.CONCLUSION:The function of retinal ganglion cells of amblyopia is damaged.PhNR by red flashes on a blue background is more sensitive than PhNR by white flashes on a white background.