目的:观察形觉剥夺性近视豚鼠巩膜组织学改变和TGF-β2在巩膜中的表达。方法:用头套形觉剥夺右眼的方法诱导近视动物模型,实验前后检测豚鼠双眼屈光状态及眼轴长度。HE染色观察后极部巩膜形态学改变,免疫组织化学法对TGF-β2在巩膜内的表达进行定性及半定量测定。结果:实验组巩膜变薄、胶原纤维排列紊乱,粗细不均,纤维间隙变大,纤维走向不一,板层结构不清;巩膜TGF-β2表达明显下调。结论:形觉剥夺性近视眼的巩膜产生一系列的退行性改变,TGF-β2是形觉剥夺性近视形成的重要因素之一。

AIM:To observe the structure changes of sclera and determine the expression of TGF-β2 in the sclera of the model.METHODS:A facemask was worn on the right eyes of guinea pigs to develop form deprivation myopia.Before and after experiment,refraction was measured using retinoscope,and the ocular axial was determined by A-scan ultrasonography.Morphologic alterations of the posterior sclera were observed by light microsope.The expression of TGF-β2 in the posterior sclera was determined by immunohistochemistry.RESULTS:Posterior sclera of myopic eyes became thinner,and collagen fibres were disordered,fibers diameters were dissimilarity,the space between the fibres was larger,the parallel structure was illegible.The immunohistochemisty showed that expression of TGF-β2 in model eyes were lower than control eyes(P<0.05).CONCLUSION:Covering eyes with facemasks is effective,simple and convenient in making myopic model.The sclera of the model eye appeared degeneration.TGF-β2 plays an important role in the course of development in form deprivation myopia.

R778.11