·AIM:To study the joint application of the two anti-glaucoma medication—pilocarpine and timolol in different ways so as to find the best method of drug delivery and formulate the rational administration schedule.·METHODS:Experimental study.A total of 36 New Zealand white rabbits were randomly divided into six groups.They were control group,pilocarpine group,timolol group,the conventional-dose group 1,the conventional-dose group 2,pilocarpine + timolol group,and timolol + pilocarpine group.The ocular hypertension model of chronic glaucoma was established.After administering the saline or first drug delivery for 10,20,30,40,60,90,120,150,180,240 minutes,the intraocular pressure was measured to compare the changes.·RESULTS:Pilocarpine + timolol group had significant effect on the intraocular pressure reducing,with statistically significant difference(P<0.05).·CONCLUSION:The way of administering pilocarpine eye drops,and 30 minutes later administering the timolol eye drops,has the best effect on controlling the intraocular pressure than others.·