·AIM:To investigate the anti-fibrosis effect of obsolete amniotic membrane for glaucoma filtering operation in rabbits.·METHODS:Trabeculectomy was performed in 60 eyes of 30 rabbits which were divided into 2 groups.In two groups,right eyes were treated with the obsolete amniotic transplantation under sclerotic flap.In group A,the left eyes were performed trabeculectomy using interferon α-2b.In group B the left eyes played the role of control which had surgery only.3 rabbits which were selected randomly in the two groups were killed 3,7,14,30,60 days after operation and eyeballs were enucleated to observe the time of decomposition of the amniotic membrane in pathologic tissue slice,the number of fibroblast and inflammatory cells were counted with computer and their difference was compared.·RESULTS:The number of inflammatory and fibroblast cells was higher than the other groups in the amniotic membrane transplantation group 3,7,14 days after operation,which showed significant difference.The decomposed time of the amniotic membrane was about 14-21 days.·CONCLUSION:Amniotic membrane immunologic rejection reaction was induced through obsolete amniotic membrane transplantion for glaucoma filtering operation in rabbits.·