目的:在激光扫描共聚焦显微镜(laserscanning confocal microscopy,LSCM)下测定小梁细胞经染料木素(genistein,Gen)作用后钙离子浓度([Ca2+]i)的变化。方法:运用组织块培养法获得三代牛眼小梁细胞,Fulo-3/AM负载后,于LSCM下动态扫描不同浓度Gen作用后胞内荧光强度变化,得出[Ca2+]i值。结果:10-6,10-5,10-4mol/LGen可使小梁细胞[Ca2+]i呈剂量依赖性降低,其中10-5mol/LGen可使小梁细胞[Ca2+]i由328.62±16.77nmol/L减少至309.69±16.57nmol/L(n=8,P<0.05)。结论:Gen可通过松弛小梁网而致房水流出阻力减小、眼压下降。

AIM:To determine the change of intracellular free Ca2+ in trabecular meshwork cells (TMC) under the influence of genistein(Gen) through laser scanning confocal microscopy(LSCM).METHODS: TMC were obtained through the method of tissue cultured. Loaded with Fluo-3/AM, the value of [Ca2+]i was obtained by dynamically scanning changes of intracellular fluorescent intensity after application of genistein with different density.RESULTS:The application of genistein (10-6,10-5,10-4mol/L, final concentration) led to an decrease in [Ca2+]i and especially 10-5mol/L genistein led to a significant decrease in [Ca2+]i: from 328.62±16.77 to 309.69±16.57 (n=8,P<0.05). CONCLUSION:Genistein with a certain density may decrease the resistance to the outflow of aqueous by relaxing TMC and lead to an decrease of IOP.

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