护网明目散对实验性糖尿病视网膜微血管内皮细胞影响的研究
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R276.7

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中国贵州省科技厅科技基金资助项目(No.黔科合丁字[2006]2066号)~~


Investigation of the effects of huwangmingmusan on the microvascular endothelial cells of experimental diabetic retinopathy
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Science and Technology Fund of Guizhou Science and Technology Department,China (No.\[2006\] 2066)

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    摘要:

    目的:探讨护网明目散对链脲佐菌素(streptozocin,STZ)诱导的糖尿病视网膜病变(diabetic retinopathy,DR)大鼠视网膜内皮细胞的影响。方法:以STZ诱导的DR大鼠分为模型组、中药组、西药组,另设一正常对照组,连续给药45d。实验结束后取大鼠眼球制备视网膜血管消化铺片,行常规PAS染色,观察各组大鼠内皮细胞情况。结果:正常组大鼠未见视网膜血管内皮细胞增生及凋亡。中药组内皮细胞数及凋亡数与正常组比较均无统计学意义(P>0.05);与模型组比较,均有明显统计学意义(P<0.01);与西药组比较,均有统计学意义(P<0.05)。结论:护网明目散能抑制实验性DR大鼠视网膜毛细血管内皮细胞增生及凋亡,对内皮细胞有保护作用。

    Abstract:

    AIM:To investigate the effects of huwangmingmusan on retinal endothelial cells of Wistar rats infected diabetic retinopathy with streptozotocin(STZ).METHODS:The Wistar rats were divided into three groups:model,Chinese medicine,and western medicine.And also,a healthy group was prepared for comparison.After successive administration for 45 days,the rats eyes were used to prepare retinal vascular digest preparations and stained with PAS routinely.The rats endothelial cells were observed.RESULTS:No proliferation and apoptosis of rat retinal vascular endothelial cells was in normal group.There were no significant differences between Chinese medicine group and the normal group in the number of endothelial cells and apoptosis(P>0.05);but there were significant differences comparing with model group and western medicine group(P<0.01,P<0.05).CONCLUSION:Huwangmingmusan can inhibit the proliferation and apoptosis of retinal capillary endothelial cells of Wistar rats infected diabetic retinopathy with STZ and have protective effect on the endothelial cells.

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肖家翔,张磊.护网明目散对实验性糖尿病视网膜微血管内皮细胞影响的研究.国际眼科杂志, 2011,11(9):1528-1530.

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