目的:离体培养人Tenon囊成纤维细胞(HTF)并观察细胞增殖能力。方法:取斜视患者手术切除Tenon囊组织进行成纤维细胞原代培养,培养的细胞通过免疫荧光染色法进行鉴定。CCK-8法描述细胞生长曲线,测定细胞活力。流式细胞术分析细胞周期,计算增殖指数。结果:通过组织块法成功培养出HTF。不同代次原代培养的HTF之间增殖能力无明显统计学差异(P>0.05)。结论:HTF在体外易于培养,经过数次传代,增殖能力稳定,是进行Tenon囊抗纤维化研究的良好靶细胞。

AIM:To culture the human Tenon’s capsule fibroblast (HTF) in vitro and observe its proliferative activity.METHODS:Human Tenon’s capsule was obtained from strabismus surgery patients.Fibroblasts were primarily cultured and identified by immunofluorescence staining.Represented by growth curve,the cell viability was detected by cell counting kit-8(CCK-8).Cell cycle was observed by flow cytometer analysis and proliferation index were calculated.RESULTS:HTF were cultured successfully from culture in vitro.There was no significant difference of proliferative activity in different passages of HTF(P>0.05).CONCLUSION:HTF is an ideal target cell in the study on anti-fibrosis of human Tenon’s capsule due to its convenience of culturing and stable proliferative activity.

R775