目的:探讨视网膜母细胞瘤(retinoblastoma,Rb)中基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)和MMP-14的表达及其与临床病程的关系。方法:应用免疫组织化学SP法检测50例Rb组织中MMP-2和MMP-14的表达;用HMIAS-2000型全自动医学彩色图像分析系统测量其平均光密度值。统计学方法比较不同临床和病理阶段Rb表达MMP-2和MMP-14的差异。结果:患者50例Rb组织切片中MMP-2阳性表达38例,占76%;MMP-14的阳性表达41例,占82%;有视神经浸润的肿瘤MMP-2和MMP-14表达水平明显高于无视神经浸润的肿瘤(P<0.01),处于眼外期的肿瘤MMP-2和MMP-14的表达水平明显高于眼内期和青光眼期的肿瘤(P<0.01);Rb中MMP-2和MMP-14的阳性表达水平呈密切相关(P<0.01)。结论:MMP-2和MMP-14的表达水平与视神经浸润和临床分期存在密切的关系,提示其表达水平与该肿瘤的浸润与发展有关。

AIM:To investigate the expression of matrix metalloproteinase-2(MMP-2)and matrix metalloproteinase-14 (MMP-14) in retinoblastoma (Rb),and their relationships with tumor development stage.METHODS:Immunohistochemitry was used to detect the expression of MMP-2 and MMP-14 in 50 cases of paraffin embedded Rb samples.Quantitative analysis of the expression of MMP-2 and MMP-14 was assessed by HMIAS-2000 Color Pathologic Analysis System.The differences of the expression of MMP-2 and MMP-14 in each clinical and pathological stage were analyzed statistically.RESULTS:Positive expression rate of MMP-2 was 76% (38 cases)and of MMP-14 was 82%(41 cases).The expression of MMP-2 and MMP-14 was significantly higher in tumors with optic nerve invasion than in tumors without optic nerve invasion (P<0.01);the expression of MMP-2 and MMP-14 was significantly higher in tumors of extra-ocular stage than in tumors of glaucomatous stage or intra-ocular stage (P<0.01);There was a statistically significant positive correlation between the expression of MMP-2 and the expression of MMP-14 (P<0.01).CONCLUSION:MMP-2 and MMP-14 exist in retinoblastoma cells.The level of MMP-2 and MMP-14 is related to optic nerve invasion and clinical stage of Rb,which suggests the expression of MMP-2 and MMP-14 could be connected to the invasion and development of tumor cells.Further research is needed for deeper understanding of the biological behavior and better evaluation of the prognosis of Rb.

R739.7