AIM:To explore the effective method for the treatment of older children with amblyopia (10-14 years old),through observing and comparing the efficacy of Zengshineng intelligent multi-dimensional training system (referred to as Zengshineng) with comprehensive treatment. METHODS: Zengshineng and comprehensive treatment methods were applied on 196 cases of 300 (150 each) eyes in older children with amblyopia. Zengshineng group was given different visual training programmes such as visual stimulation, fine visual training. Comprehensive treatment group was given treatments such as cover, fine visual training,CAM visual stimulation,lights flashing, ocular spectrum,light brush.The best-corrected visual acuity of two groups in the treatment of amblyopia after 1 year was compared.The older children with amblyopia without binocular visual function began to train their three-level visual function after the best-corrected visual acuity reaching 0.6，Zengshineng group by multidimensional three-level function training and comprehensive treatment group by Shi-Jia amblyopia therapy instrument 400 additional three-level function training，observing the binocular visual function changes after 6 months. RESULTS:Mild amblyopia efficiency of Zengshineng group and comprehensive treatment group was close and no statistically significant difference between the two groups(P＞0.05). Moderate amblyopia and severe amblyopia efficient difference between the two groups had statistically significant difference(P<0.05). Zengshineng group had significantly higher efficiency than comprehensive treatment group. Zengshineng group obtained near stereopsis with a higher efficiency than the comprehensive treatment group among the children with amblyopia when best-corrected visual acuity reached 0.6 and no binocular visual function after three-level visual function training for 6 months(P<0.05). CONCLUSION:Zengshineng intelligent multi-dimensional training system is an effective method to treat moderate and severe amblyopia for older children.