AIM:To observe clinical curative effect of esculin and digitalisglycosides eye drops in the treatment of visual display terminals(VDT) syndrome. METHODS:Totally 50 cases ( 100 eyes ) on the VDT syndrome were treated with esculin and digitalisglycosides eye drops for 30 days, 9 eyestrain symptoms scores were observed before and after treatment. RESULTS:Of the 50 cases ( 100 eyes), symptom mean total score before treatment was 12.64±6.56, after treatment was 3.56±4.80, the difference was statistically significant（t=8.7409，P＜0.01）.during the period of observation there was no treatment adverse reaction. CONCLUSION:Esculin and digitalisglycosides eye drops in the treatment of VDT syndrome is effective and safe to recently alleviate eyestrain symptoms.