AIM:To observe the clinical effect of natamycin joint 5g/L povidone iodine for treatment of fungal corneal ulcer. METHODS:Totally 38 cases (38 eyes) of fungal corneal ulcer used natamycin joint 5g/L povidone iodine and related antifungals (Sporanox, etc.) for treatment, and were analyzed retrospectively. RESULTS:After treatment with natamycin joint 5g/L povidone iodine and Sporanox (itraconazole) and other drugs, 30 eyes cured, 7 eyes improved, 1 eye lost eyeball due to corneal perforation and fungal endophthalmitis.The cure rate was 79%, effective rate was 97%. CONCLUSION: Natamycin joint 5g/L povidone iodine and Sporanox (itraconazole) and other drugs for treatment of fungal corneal ulcer is safe and effective, simple and practical.