目的：观察反义CD44基因转染对人眼小梁细胞合成细胞外基质的影响，探讨黏附分子CD44在原发性开角型青光眼(primary open angle glaucoma, POAG)发病过程中可能的作用。 方法：采用硫代修饰的CD44反义寡核苷酸，通过脂质体介导转染体外培养的人眼小梁细胞，免疫组织化学染色观察CD44反义寡核苷酸对小梁细胞合成胶原蛋白Ⅰ型、层黏附蛋白的影响，放射免疫法观察CD44反义寡核苷酸对小梁细胞合成透明质酸的影响。 结果：CD44反义寡核苷酸对人眼小梁细胞合成胶原蛋白Ⅰ型、层黏附蛋白起促进作用，且呈浓度依赖性，浓度越高促分泌作用越强,但对小梁细胞合成透明质酸起抑制作用，浓度越高抑制作用越明显。 结论：CD44反义寡核苷酸封闭CD44基因表达后人眼小梁细胞合成胶原蛋白Ⅰ型、层黏附蛋白增加而合成透明质酸减少。黏附分子CD44可能通过影响小梁细胞合成细胞外基质功能参与了POAG发病过程。

To observe the effect of CD44-specific antisense oligonucleotide on extracellular matrix（ECM） synthesis of human trabecular meshwork cells, then to discuss the possible relationship between CD44 and primary open angle glaucoma（POAG）. METHODS: CD44-specific antisense oligonucleotide were delivered with cationic lipid to cultured human trabecular meshwork cells. Effect of CD44-specific antisense oligonucleotide on synthesis of collagen-Ⅰ, Laminin were examined by immunohistochemistry and the synthesis of hyaluronic acid were examined by radioimmunoassay. RESULTS: Three different concentrations of CD44 antisense oligonucleotide could promote the synthesis of collagen-Ⅰ, Laminin in trabecular meshwork cells and in a concentration-dependent manner. But CD44 antisense oligonucleotide inhibited the synthesis of hyaluronic acid. CONCLUSION: CD44 antisense oligonucleotide promotes the synthesis of collagen-Ⅰand Laminin but inhibits the synthesis of hyaluronic acid. CD44 may play a role in pathogenesis of POAG by affecting the synthesis of ECM.

中国青海省自然科学基金资助项目（No.2009-Z-706）