To investigate the application of 23-Gauge (23G) vitrectomy system in high altitude area. METHODS:In the applying of 23G vitrectomy system, the vitrectomy had been done for 32 cases (32 eyes), including vitreous hemorrhage, macular hole, macular pucker, posterior dislocation of lens, retinal detachment, Tersonz syndrome, vitreous hemorrhage combining with retinal detachment. RESULTS:The operations were successful, without expanding sclera puncture.The postoperative syndrome mainly was low IOP. CONCLUSION:In 2000, the clinical using of 23G vitrectomy system without conjunctival suture was first reported. It is more efficient than 25G vitrectomy system, which can process the retina around like 20G and has the advantage of healing fast ，seamless， short operation time and less postoperative syndrome as 25G vitrectomy system. So it can be widely applied in the vitrectomy in high altitude area.