方法：体外培养从角膜移植术后新鲜人眼球提取的HRCECs。取生长良好的第 3～4代细胞用于实验，实验分为低糖对照组、高糖对照组、高糖+(60，80，100，120μg/mL)不同浓度莪术油组，用噻唑蓝(MTT)比色法检测HRCECs的增殖，通过免疫细胞化学法观察各分组HRCECs中VEGF的表达情况。

结果：MTT比色法结果显示：高糖对照组与低糖对照组没有显著差异(P>0.05)，用60，80，100，120μg/mL的莪术油处理高糖下HRCECs作用24h高糖+不同浓度莪术油组与高糖对照组相比具有显著性差异(P<0.05)，高糖+不同浓度莪术油组之间呈时间和浓度的显著性差异(P<0.05)。免疫细胞化学检测显示：与低糖对照组相比，高糖对照组VEGF表达明显(P<0.05)，用80，100，120μg/mL的莪术油处理高糖下HRCECs 24h，高糖+不同浓度莪术油组与高糖对照组相比VEGF表达有显著性差异(P<0.05)，高糖+不同浓度莪术油组之间两两比较均具有显著性差异(P<0.05)。

结论：莪术油可抑制高糖下人视网膜血管内皮细胞增殖和VEGF表达。

METHODS: Fresh eyeballs HRCECs extracted just after cornea transplanting operation were cultivated in vitro. The third or fourth generation healthy cells for this experiment were obtained, and divided into three groups: low glucose, high glucose, high glucose plus different density of Rhizoma Zedoariae group(60, 80, 100, and 120μg/mL). MTT was employed to detect the multiplication of HRCECs, and the HRCECs VEGF through immune cells was observed.

RESULTS: MTT comparison: there was no obvious difference between high glucose and low glucose groups(P>0.05), while there was significant difference between high glucose+ and high glucose groups(P<0.05), and different density of glucose showed big difference. Immune cells: high glucose group had obvious affection compared with low glucose group, and there was significant difference between high glucose+ group and high glucose group in their affection on VEGF(P<0.05), as well as in different density among high glucose group.

CONCLUSION: Rhizoma Zedoariae can restrain the multiplication of HRCECs and VEGF in high glucose environment.