AIM:To build up the model of experimental choroidal neovascularization(CNV), for preparing the further pharmacodynamics experiment.

METHODS:The method of subretinal injection of Matrigel on grey rabbits was used to build up the model of experimental CNV. Fundus examination, FFA, choroidal flat mount and pathological changes were measured to choose the best dose and injecting technique of Matrigel.

RESULTS: In the FFA examination, the fluorescence leakage was happened in Matrigel 40μL and 20μL groups at 7 day, which was enhanced at 14 day and 28 day; the fluorescence leakage was happened in Matrigel 10μL group at 14 day and 28 day,which was never found in Matrigel 5μL group. In the fundus examination, most of retinas were attached back at 7 day; a few of those were detached. A few of retina had proliferative detachment in Matrigel 40μL group. Cataract and vitreous muddy were found in Matrigel 40μL group. In the tissue pathological examination, small vessels and/or immature fibrous tissues were found in Matrigel 40μL and 20μL groups. The retinal injure was most severely in Matrigel 40μL group.

CONCLUSION: The method of subretinal injection of Matrigel could create CNV successfully, 20μL is the best dose.