AIM: To explore the protective effects of cattle encephalon glycoside and ignotin(CEGI)on developmental process of rds mice, investigate its function and effects on retinal degenerative diseases, and offer the basis on treating similar diseases.

METHODS:The retinal pathologic and ultrastructure changes of rds mice treated by CEGI were observed by light and electron microscopy, and the apoptosis rates of photoreceptor cells were determined through TUNEL(tdt- mediated biotin-dUTP nick-end labeling)technique.

RESULTS: Postnatal 14 days(P14)was the best stage of developmental process of rds mice. After 28-56 days, cell number on retinal outer nuclear layer and retinal inner layer reduced, and photoreceptor apoptosis increased gradually. What's more, electron microscopy showed apoptosis nuclear changes and disintegration of inner section and cilia. On 28 days and 56 days, CEGI groups showed thicker retinal cell layers and less apoptotic cells compared with the control group. There was no statistically significant difference in comparison. Nerve growth factor(NGF)had similarity function with CEGI.

CONCLUSION: CEGI can promote growth and development of retinal cells, and have a protective effect on retinal degenerative process of rds mice.