多西环素对碱烧伤大鼠角膜炎症细胞浸润的抑制作用
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国家自然科学基金(No.81160119); 广西自然科学基金(No.2012GXNSFAA053107); 广西高等学校立项科研项目(No.201204LX064)


Doxycycline inhibits the infiltration of inflammatory cells in rat alkali burn corneas
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National Natural Science Foundation of China(No.801160119); Natural Science Foundation of Guangxi Zhuang Autonomous Region, China(No.2012GXNSFAA053107); Foundation for University Teacher of Guangxi(No.201204LX064)

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    摘要:

    目的:观察多西环素对碱烧伤大鼠角膜炎症细胞浸润的抑制作用。

    方法:健康SD大鼠32只,随机分为对照组、多西环素组,每组16只大鼠。建立角膜碱烧伤模型后,多西环素组予3g/L多西环素眼液点眼,对照组予眼液溶媒点眼。分别于碱烧伤后第3,7,14,21d观察计算炎症指数,角膜取材后进行病理切片及炎症细胞计数,行ICAM-1的ELISA检测。

    结果:多西环素组各时间点结膜充血、角膜水肿较对照组轻,炎症指数均明显低于对照组,差异有统计学意义(P< 0.05)。多西环素组各时间点炎症细胞计数少于对照组(P< 0.05)。多西环素组各时间点大鼠角膜的ICAM-1表达低于对照组(P<0.05)。

    结论:多西环素可以抑制碱烧伤大鼠角膜的炎症细胞浸润,其机制可能是抑制ICAM-1的表达。

    Abstract:

    AIM: To investigate the inhibited effect of Doxycycline on the infiltration of inflammatory cells in rat alkali burn corneas.

    METHODS: Corneas of 32 SD rats were injured with 1mol/L NaOH, then divided into two groups: control and doxycycline-treated. All agents were administered topically 4 times daily. Slit-lamp microscope was performed and inflammatory index was calculated at 3, 7, 14 and 21 days after injury. Then 4 rats were randomly sacrificed and each cornea was divided into two parts, one for histopathology, the other for ICAM-1 ELISA assay.

    RESULTS:In control group, the inflammatory index and the number of inflammatory cell was higher than the doxycycline-treated dramatically at all time points(P<0.05). Compared with control group, corneal ICAM-1 expression decreased significantly in doxycycline-treated group at all time points(P < 0.05).

    CONCLUSION:Doxycycline may inhibit inflammatory cell infiltration by down-regulating ICAM-1 expression.

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赵静博,邹文进,付馨余.多西环素对碱烧伤大鼠角膜炎症细胞浸润的抑制作用.国际眼科杂志, 2013,13(6):1090-1092.

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  • 收稿日期:2013-01-29
  • 最后修改日期:2013-05-27
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  • 在线发布日期: 2013-06-03
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