方法：采用Western-blot和RT- PCR的方法检测60只雌鼠泪腺中MMP1和TIMP1的表达情况，并进行SⅠt检测。

结果：雌鼠去势后SⅠt显著缩短，MMP1和TIMP1蛋白、mRNA表达均显著增高(P<0.05)； 给予雌激素后，SⅠt比给药前显著缩短，MMP1和TIMP1蛋白、mRNA表达较给药前均显著增高(P<0.05)。

结论：雌激素上调MMP1和TIMP1表达，而且可以加重泪液分泌的减少，所以临床需慎用雌激素治疗绝经后干眼症。

METHODS:A total of 60 female rats were examined for the expression of MMP-1 and TIMP-1 protein in lacrimal gland through SⅠt measurement together with Western- blot and RT-PCR methods.

RESULTS:There was significant reduction in SⅠt and increase in the protein expression of MMP1, TIMP1 and mRNA after ovariectomy conducted on rats(P<0.05). After estrogen treatment, there was significant reduction in SⅠt and increase in the expression of MMP1, TIMP1 and mRNA as compared with before treatment(P<0.05).

CONCLUSION: Estrogen must be applied cautiously to treat xerophthalmia following menopause because estrogen can up regulate the expressions of MMP-1 and TIMP-1 and can aggravate the decrease of lacrimal secretion.