方法：用培养的供体来源的imDC及IL-10-imDC预处理受体，建立Wistar-SD大鼠穿透性角膜移植模型。供体Wistar大鼠18只； 受体SD大鼠36只，受体鼠随机分为3组，每组均为12只。A组(对照组)：受体鼠不经任何预处理即行角膜移植术； B组：角膜移植前3d，每只受体鼠经尾静脉注射供体的imDC，细胞数为2×10⁶个； C组：角膜移植前3d，每只受体鼠经尾静脉注射用IL-10修饰的供体imDC，细胞数为2×10⁶个。观察术后各组受体角膜植片的存活时间； 术后第14d各组随机抽取4只鼠取术眼角膜行组织病理学检查及免疫组化法检测NF-κB的表达。

结果：三组植片存活时间分别为10.17±2.16，19.83±2.25，27.57±1.72d； 三组组间比较，差异均有显著性(P<0.01)。A组角膜植片显著水肿、增厚，出现大量的淋巴细胞和单核细胞浸润，角膜基质排列紊乱。B，C两组植片炎性细胞浸润均显著减少，角膜厚度及结构基本正常。NF-κB在A组即对照组中呈高表达(P<0.05)，B，C组间比较无显著性差异(P>0.05)。

结论：IL-10可有效抑制树突状细胞(dendritic cells，DC)成熟。摄取供体的imDC能显著延长角膜植片的存活时间。IL-10-imDC可抑制NF-κB的表达水平，从而抑制角膜排斥反应的发生。

METHODS: Eighteen Wistar rats were used as donors and 36 SD rats were used ad recipients. The recipient was injected by tail with donor IL-10-treated imDC or with untreated imDC. The rats were randomly assigned to three groups, 12 in control group received nopretreatment, 12 in imDC group were injected with imDC(2×10⁶)3d before surgery, and 12 in IL-10-imDC group were injected with IL-10-imDC(2×10⁶)3d before surgery. The survival time of corneal allografts in three groups were observed. The expression of NF-κB was detected by histopathological examination 14d postoperative.

RESULTS: The mean survival time was merely 10.17±2.16d, 19.83±2.25d and 27.57±1.72d respectively in control, imDC and IL-10-imDC groups. There was significantly difference among three groups(P<0.01). Corneas in groups B and C had fewer infiltrating cells, corneal thickness almost normal, and stroma arrangement in order, the center of the grafts showed no inflammatory cellular infiltration. NF-κB cells strongly expressed in control group(P<0.05), and there was no significant difference between groups B and C(P>0.05).

CONCLUSION: IL-10 can restrained DC from mature. Derivatives from donor imDC can markedly prolong the survival time of corneal allografts. NF-κB may play a potential role in the development of corneal graft rejection.