方法：新西兰大白兔45只，随机分为(A，B，C，D，E)五组恒温烧灼器诱导实验性CNV模型，除E组为5只，其他组均为10只。A组：100℃，B组：200℃，C组：300℃，D组：400℃，E组：空白对照。以左眼为实验眼，比较建模后第4，7，14，30d在裂隙灯显微镜下观察各组角膜新生血管的生长情况并计算角膜新生血管生长面积。采用SPSS 19.0统计软件包对数据进行分析，计算资料用(-overx)±s表示，各时间点各自新生血管面积的比较采用重复测量数据的方差分析； 显著性标准为α=0.05。

结果： 制模后第4，7，14，30d，A组角膜热烧伤后无明显新生血管生长，仅少量留有角膜云翳(n=2)。B组新生血管面积分别为：9.16±1.45，37.73±5.49，62.44±7.54，40.28±7.39mm²； C组新生血管面积分别为：11.45±1.04，44.51±4.64，66.13±4.13，43.04±2.33mm²； D组新生血管面积分别为：13.23±0.86，47.26±4.59，67.57±4.56，45.59±4.44mm²； D组部分角膜局部出现焦化(n=4)，3d内出现穿孔(n=6)。E组未见新生血管生长。各时间点的CNV面积比较，差异有显著性(P<0.05)，B，C，D分组之间有统计学差异(P<0.05)。

结论：角膜热烧伤4～7d时，制模温度越高，新生血管生长越快，面积越大； 14～30d新生血管面积无明显差别，但400℃角膜制模失败率高，200℃～300℃制作的兔角膜新生血管模型均一性及重复性高。

METHODS: Total 45 New Zealand white rabbits were divided randomly into five groups(A, B, C, D, E). A groups: 100℃(n=10), B groups: 200℃(n=10), C groups: 300℃(n=10), D groups: 400℃(n=10), and E groups: control group(n=5). All left eyes of rabbits in A,B,C,D groups were induced corneal neovascularization by constant temperature burning device. The growth of CNV was observed by slit lamp microscope and the area of CNV were recorded on 4 ^th, 7 ^th, 14^th, 30^th days postoperatively. SPSS 19.0 statistical package was used for data analysis, and the data was recorded by mean±standard deviation. Comparison by analysis of variance was made by repeated measures in the area of neovascularization at each time point in groups. Statistical tests were considered significantly when P values were less than 0.05.

RESULTS: On postoperative 4^th, 7^th, 14^th, 30^th days: no neovascularization was found after corneal thermal burn in A group, but only a few nebula left(n=2); the area of CNV were(9.16±1.45)mm²,(37.73±5.49)mm²,(62.44±7.54)mm²,(40.28±7.39)mm² in B group respectively; and(11.45±1.04)mm²,(44.51±4.64)mm²,(66.13±4.13)mm²,(43.04±2.33)mm² in C group respectively; and(13.23±0.86)mm²,(47.26±4.59)mm²,(67.57±4.56)mm²,(45.59±4.44)mm² in D group respectively, and part corneal carbide(n=4)was observed as well as corneal perforation(n=6)were found on 3d in D group. No neovascularization was found in normal control group. Comparison of the areas of CNV at each time point between groups was statistically different, P<0.05. Statistical differences were found among B, C, D groups, P<0.05.

COCLUSION: In 4 to 7d, the higher the temperature is, the more the neovascularization area of CNV are. It has no significant difference in 14 to 30d. But corneal carbide and corneal perforation are often found in 400℃ group, so its modeling failure rate is high. It is between 200℃ and 300℃ that repeatability and uniformity of the corneal neovascularization model of rabbit are superior.