目的：通过建立大鼠糖尿病视网膜病变(diabetic retinopathy，DR)模型，观察造模后大鼠的血糖以及视网膜和胰腺组织的病理变化，初步评价双丹明目胶囊对改善DR视网膜和胰腺组织结构的效应。

方法：将双丹明目胶囊作用于DR大鼠，通过与正常组、模型对照组、阳性对照组的比较，观察双丹明目胶囊对DR大鼠视网膜和胰腺形态学的影响。

结果：经双丹明目胶囊治疗2mo后，双丹明目组大鼠血糖和糖化血红蛋白与模型对照组比较均明显降低，血糖差异有显著意义(P<0.01)。双丹明目组病变较模型对照组轻，光感受器细胞层排列基本整齐，各层组织细胞内、细胞外水肿现象较模型对照组轻，毛细血管管腔无闭塞，周细胞轻度水肿。

结论：双丹明目胶囊能有效降低DR大鼠血糖和糖化血红蛋白含量。双丹明目胶囊能有效改善DR大鼠视网膜和胰腺组织结构。

AIM: To observe pathological changes of blood glucose, the retina and pancreatic tissue after establishing rats diabetic retinopathy(DR)model, and preliminary evaluate of Shuangdanmingmu capsule on the improvement of retina and pancreas tissue structure.

METHODS: DR rats were fed with Shuangdanmingmu capsule. Then the blood glucose and glycated hemoglobin were determined. Immunohitochemical staining was used to observe the expression of structure of retino and pancreas.

RESULTS: Compared with the control group, the blood glucose and glycates hemoglobin in Shuangdanmingmu group were decreased after 2mo of treatment(P<0.01). The layers tissue of retina was less edema and gradually arranged in Shuangdanmingmu group. The structure of pancreas was clear basically. Fat cells, infiltrati on of lymphocytes and fibrosis hyperplasia appeared in the interacinar and interlobular connective tissue. Some acinar and islet cells of fatty rat pancreatic tissue had fatty or vesicular degeneration.

CONCLUSION: Shuangdanmingmu capsule can decrease blood glucose and glycates hemoglobin in DR rats effectively and improve the ultrastructure of retina and pancreas tissue.

国家自然科学基金面上项目(No. 81473737); 湖南省教育厅科研基金重点项目(No.13A072)