方法：按照时间先后顺序，采用DCD供体角膜行穿透性角膜移植术，术后3～4mo检测角膜植片内皮细胞密度及最佳矫正视力。

结果：共统计14例26眼DCD角膜供体纳入本研究。供体年龄0.5～61(平均38.3±15.6)岁； 死亡原因包括脑外伤9例，心肌梗塞2例，脑干出血2例，呼吸循环衰竭1例。14例DCD捐献者28眼，因2眼暴露性角膜炎用作科学研究，共26眼用于穿透性角膜移植。所有患者手术过程顺利，手术后3～4mo未发生排斥反应，角膜植片保持透明。手术后3～4mo角膜内皮细胞密度794～4 347(平均2 305±827)个/mm² ，其中低于1 000个/mm²者1例(3.8%)，1 000～2 000个/mm²者9例(34.6%)，大于2 000个/mm²者16例(61.5%)。26例角膜移植受者年龄20～80(平均40.7±17.1)岁。手术前最佳矫正视力为光感(+)～0.08(平均0.027±0.024)； 手术后3～4mo最佳矫正视力为0.2～0.8(平均0.52±0.182)，两者比较差异有显著统计学意义(t=3.96，P<0.01)。

结论：DCD捐献角膜用作穿透性角膜移植供体具有较高的安全性。

METHODS: In chronological order, suing DCD corneas penetrating keratoplasty, corneal endothelial cell density and best corrected visual acuity(BCVA)were tested 3～4mo after surgery.

RESULTS: A total of 14 cases of DCD while 26 corneas were included in this study. Donors age ranged 0.5～61 years, averagely 38.3±15.6 years. Causes of death included that 9 cases of traumatic brain injury, 2 cases myocardial infarction, 2 cases brain stem hemorrhage, 1 case of respiratory and circulatory failure. All 26 patients underwent penetrating keratoplasty, no rejection occurred and all grafts were transparent 3～4mo after surgery. Three to four months after surgery, corneal endothelial cell density ranged 794～4 347/mm², averaged 2 305±827/mm², within which was only one case was lower than 1 000/mm²(3.8%), while 9 cases ranged from 1 000～2 000/mm²(34.6%), 16 cases were higher than 2 000/mm²(61.5%). The age of all the 26 receipts were from 20～80 years, mean 40.7±17.1 years. BCVA before surgery was light perception positive to 0.08, with an average 0.027±0.024. Three to four months after surgery, BCVA were 0.2～0.8, with an average 0.52±0.182 in contrast(t=3.96, P<0.001).

CONCLUSION: DCD donated corneas could be used for penetrating keratoplasty graft with high security.