Abstract:AIM: To investigate theprotective effect of melatonin against hydrogen peroxide(H2O2)-induced oxidative damage to human lens epithelial cells.
METHODS: Sub-culture human lens epithelial cells preprocessed with different concentrations of melatonin for 12h and then 100 μmol/L H2O2 for 24h. The impact of melatonin on H2O2-induced lens epithelial cell viability was detected by MTT assay, rate of apoptosis was detected by flow cytometry instrument and activity of apoptosis-related factors, Caspase-3 and Caspase-9, were detected by colorimetric method.
RESULTS: MTT assay showed that melatonin had no effect on the activity of lens epithelial cells, and the drug can inhibit the decrease of H2O2-induced cell activity, as well as flow cytometry showed that melatonin can inhibit H2O2-induced apoptosis. In addition, melatonin can also reduce H2O2-induced Caspase-3 and Caspase-9 activity in lens epithelial cells, and their activity decreased with effect of melatonin along with extending time.
CONCLUSION: Melatonin can obviously inhibit H2O2-induced apoptosis of human lens epithelial cells, which provide reliable experimental basis for drug on treatment of cataract.
