方法：将300例原发性闭角型青光眼患者作为观察对象，同时随机选择300例健康志愿者的血液标本作为对照，并将观察组患者分为慢性、急性两亚组，采用PCR技术检测GSTM1和GSTT1基因多态性。

结果：GSTT1缺失基因型在观察组中的分布频率为54.3%，在对照组的分布频率为54.0%，两组差异无统计学意义(χ²=0.053，P>0.05)； 观察组中慢性组GSTT1缺失基因型分布频率为54.9%，急性组GSTT1缺失基因型分布频率为48.6%，急性组与对照组相比，差异无统计学意义(χ²=0.064，P>0.05)，慢性组与对照组差异无统计学意义(χ²=0.037，P>0.05)； 观察组GSTM1缺失基因型分布频率为59.0%，对照组为55.7%，两组差别无统计学意义(χ²=0.013，P>0.05)； GSTM1缺失基因型在观察组急性和慢性两亚组的分布频率分别为62.3%和58.1%，慢性组与对照组差异无统计学意义(χ²=0.047，P>0.05)，急性组与对照组差异无统计学意义(χ²=0.005，P>0.05)。

结论：原发性闭角型青光眼易感性与GSTM1和GSTT1基因多态性间不存在明显相关性。

METHODS: Totally,300 cases were collected from primary angle-closure glaucoma patients and 300 health volunteers were served as control group. The observation group were divided into chronic and acute primary angle-closure glaucoma groups, then multiplex PCR technology was used to detect the genetic polymorphisms of GSTM1 and GSTT1.

RESULTS:The distribution frequencies of GSTT1-null genotype were 54.3%, while it was 54.0% in the control group, statistically no significance between control group and observation group(χ²=0.053, P>0.05); The frequency GSTT1-null genotype in chronic group of primary angle-closure glaucoma was 54.9%, while it was 48.6% in the acute group of primary angle-closure glaucoma, statistically no significance between control group and acute group(χ²=0.064, P>0.05), and chronic group(χ²=0.037, P>0.05); The distribution frequencies of GSTM1-null genotype was 59.0%, while it was 55.7% in the control group, statistically no significance between control group and observation group(χ²=0.013, P>0.05); The distribution frequencies of GSTM1-null genotype was 62.3% and 58.1% in acute and chronic group of primary angle-closure glaucoma patients respectively. Acute group of primary angle-closure glaucoma has no significant compared with control group(χ²=0.005, P>0.05), Chronic group of primary angle-closure glaucoma had no significant compared with control group(χ²=0.047, P>0.05).

CONCLUSION:No statistic significance relationship is found between primary angle-closure glaucom patients and the null genotypes of GSTM1 and GSTT1 susceptibility.