方法：取健康6～8wk Balb/c小鼠30只，随机分成正常对照组、光照组、α-倒捻子素组，每组10只。α-倒捻子素组小鼠每天接受α-倒捻子素30mg/(kg·d)灌胃7d，于灌胃给药第5d进行光损伤造模。对光照组和α-倒捻子素组小鼠采用5 000±200lx白色LED光连续照射1h。模型完成后72h记录各组小鼠闪光视网膜电图； 应用光镜观察各组小鼠视网膜形态学变化； 剥离视网膜，检测各组小鼠视网膜组织匀浆中丙二醛(MDA)含量。

结果：闪光视网膜电图(F-ERG)显示α-倒捻子素组视网膜功能损伤较光照组明显减轻(P<0.05)。光镜下，α-倒捻子素组视网膜结构损伤相较于光照组明显减轻(P<0.05)。相对于光照组，α-倒捻子素组视网膜组织MDA含量明显减少(P<0.05)。

结论：α-倒捻子素抑制光损伤引起的视网膜脂质过氧化，对小鼠视网膜光损伤起到保护作用。

METHODS: Totally 30 Balb/c mice, aged 6～8wk, were randomly divided into the control group, light-exposure group and α-mangostin group. Every group contained 10 mice. Mice of α-mangostin group were treated with alpha-mangostin at the dose of 30mg/(kg·d)body weight by intragastric administration daily for 7d, and then exposed to white light at the 5^thd. The light-exposure group and α-mangostin group were exposed to 5 000±200lx white light-emmiting diodes(LEDs)for continuously 1h to establish the mice model of retinal light damage. Flash-electroretinograme was recorded 72h after light exposure. The changes in retinal morphology of mice were observed by light microscopy. Retinas were extracted to detect the malondialdhyde(MDA)content change of the retinal homogenate.

RESULTS: Flash-electroretinogram(F-ERG)showed that retinal dysfunction was less severe in α-mangostin group than in light-exposure group(P<0.05). Light microscopy test showed that retina structural damage was less severe in α-mangostin group than in light-exposure group(P<0.05). The level of MDA in retinal tissue of α-mangostin group was significantly lower when compared with light-exposure group(P<0.05).

CONCLUSION: α-mangostin inhibits lipid peroxidation induced by light damage and protect retina against light damage.