方法：选取2012-03/2014-10本院收治的复发性翼状胬肉患者15例作为本次研究的实验组，另选取同期15例单纯性胬肉患者作为对照组。通过免疫组化Envision法对两组患者的手术标本中的iNOS与CD34检测，并计算微血管密度(microvessel density，MVD)，比较两组患者的iNOS与CD34阳性表达率和MVD值，分析iNOS和CD34对复发性翼状胬肉的影响作用。

结果：对照组中15例单纯性胬肉患者iNOS阳性表达率为7%(1例)，实验组中15例复发性翼状胬肉患者iNOS阳性表达率为87%(13例)； 对照组CD34阳性表达率为13%(2例)，实验组CD34阳性表达率为80%(12例)。iNos与CD34在复发性翼状胬肉患者中较结膜正常人群中的阳性表达率明显增高，且均存在显著性差异(P<0.01)。实验组MVD值为46.02±10.88，对照组MVD为23.48±5.68，实验组MVD值明显高于对照组且两组存在统计差异(P<0.01)。

结论：iNOS与CD34在复发性翼状胬肉患者中阳性显著表达率高，说明iNOS与CD34与复发性翼状胬肉疾病的发生、发展存在一定联系。

METHODS:From March 2012 to October 2014, 15 patients with recurrent pterygium were treated as the experimental group of this study and 15 cases with simple pterygium were selected as the control group. iNOS and CD34 in two groups of patients were detected by immunohistochemical Envision method. Microvessel density(MVD)was calculated. The positive expression rates of iNOS and CD34 and MVD in the two groups were compared, and the effect of iNOS and CD34 on recurrent pterygium was analyzed.

RESULTS:The positive expression rate of iNOS was 7%(1 case)in the control group and 87%(13 cases)in the experimental group. The positive expression rate of CD34 in control group was 13%(2 cases)and was 80%(12 cases)in the experimental group. The positive expression rate of iNOS and CD34 in the patients with recurrent pterygium were significantly higher than those in the normal group(P<0.01). The MVD value of the experimental group was 46.02±10.88, and the control group MVD was 23.48±5.68. The MVD of the experimental group was significantly higher than that of the control group and the difference between the two groups was statistically significant(P<0.01).

CONCLUSION:iNOS and CD34 were significantly higher in the patients with recurrent pterygium. The relationship between iNOS and CD34 was related to the occurrence and development of recurrent pterygium.