[关键词]
[摘要]
目的:研究羊膜提取液对苯扎氯铵诱导的兔干眼的治疗作用。
方法:选择新西兰大白兔26只(26眼,均为右眼),使用0.2%的苯扎氯铵制作兔干眼症模型,随机分为两组:对照组(A组,13眼)使用磷酸盐缓冲液(phosphate buffered saline,PBS)滴眼,实验组(B组,13眼)使用1%羊膜提取液(amniotic extraction)滴眼,4次/d。另选取2只兔作为正常对照。分别于治疗前和治疗1、2、4、8wk行Schirmer I试验(Schirmer Ⅰ test,SⅠt)、角膜荧光素染色(corneal fluorescein staining,FL)、泪液总蛋白含量、淀粉酶活性、乳铁蛋白、溶菌酶含量检测,并进行结膜印迹细胞检查。
结果:不同组间、不同时间点间SⅠt、FL、溶菌酶活性及结膜杯状细胞密度相比,差异有统计学意义(P<0.05)。治疗前两组SⅠt、FL、溶菌酶活性及结膜杯状细胞密度相比,差异无统计学意义(P>0.05); 治疗1、2、4、8wk后,B组SⅠt、FL、溶菌酶活性较治疗前均有不同程度改善,差异有统计学意义(P<0.05),B组结膜杯状细胞密度与治疗前相比有所增加,但差异无统计学意义(P>0.05); 治疗1、2、4、8wk后,A组SⅠt、溶菌酶活性及结膜杯状细胞密度均有不同程度下降,差异有统计学意义(P<0.05),A组FL评分与治疗前相比有不同程度增加,但差异均无统计学意义(P>0.05)。A、B两组各时间点SⅠt、FL、溶菌酶活性及结膜杯状细胞密度相比,差异均有统计学意义(P<0.05)。同一组内不同时间点,泪液中总蛋白量、乳铁蛋白、淀粉酶活性差异无统计学意义(P>0.05)。治疗前A、B两组泪液中总蛋白量、乳铁蛋白、淀粉酶的活性相比,差异无统计学意义(P>0.05); 治疗4、8wk后两组泪液中总蛋白量、乳铁蛋白、淀粉酶的活性相比,其差异有统计学意义(P<0.05)。
结论:羊膜提取液能有效治疗兔干眼症,具有良好的临床应用前景。
[Key word]
[Abstract]
AIM: To investigate therapeutic efficiency of amniotic extraction on dry eye in rabbit model induced by topical benzalkonium chloride(BAC).
METHODS: Totally 26 rabbits(26 right eyes)with dry eye model were studied and divided into two groups: group A(control group with PBS eye drops, n=13)and group B(amniotic extraction group, n=13). Another two rabbits were chosen as normal control.The Schirmer Ⅰ tests(SⅠt)and corneal fluorescein staining(FL)were made, and the tear total protein content, amylase activity, lactoferrin, lysozyme contents, goblet cell density were performed in two groups before treatment and 1, 2, 4 and 8 wk after treatment.
RESULTS: There were significant differences in SIT, FL scores, lysozyme activity and goblet cell density among different groups at different time points(P<0.05). But, there was no significant differences in SⅠt, FL scores, lysozyme activity and goblet cell density between two groups before treatment(P>0.05). After 8wks' treatment with PBS, the mean differences of the group A showed great changes in SⅠt, lysozyme and goblet cell density compared with those before treatment(P<0.05); but there was no significant differences in FL scores compared with those before treatment(P>0.05). As for group B, 8wks after treatment, there were statistical changes in SⅠt, FL, lysozyme(P<0.05); but there was no significant differences in goblet cell density compared with those before treatment(P>0.05). It was evident that statistical differences were observed in SⅠt, FL scores, lysozyme activity and goblet cell density between two groups at each time point(P<0.05). However, there were no significant differences in total protein, lactoferrin, amylase activity at different time points(P>0.05). Meanwhile there was no significant differences in total protein, lactoferrin, amylase activity between two groups before treatment(P>0.05). But there were significant differences in total protein, lactoferrin, amylase activity between two groups after 4 and 8 wks' treatment(P<0.05).
CONCLUSION: Amniotic extraction has significant therapeutic effect on the dry eye in rabbit model.
[中图分类号]
[基金项目]
国家自然科学基金资助项目(No.81160118,81400372); 佛山市卫生和计生局医学科研项目(No.2015306)