方法：选取本院诊断治疗的孔源性视网膜脱离患者48例52眼，根据视网膜脱落范围分<1/2象限组，1/2～3/4象限组和>3/4象限组，选取同期在我院健康体检者55例55眼作为对照组，比较孔源性视网膜脱离患者与对照组的血清中氨基酸和VEGF水平的差异。比较孔源性视网膜脱离患者不同脱离程度视网膜下液中氨基酸以及VEGF水平的差异。视网膜下液中VEGF水平与氨基酸的相关分析。

结果：孔源性视网膜脱离患者血清中色氨酸28.59±4.46mg/L、苯丙氨酸8.95±2.55mg/L、蛋氨酸8.15±2.17mg/L、缬氨酸28.62±5.29mg/L、组氨酸18.96±1.85mg/L以及血管内皮生长因子589.92±185.34μg/L高于对照组，且差异有统计学意义(P<0.05)； 孔源性视网膜脱离患者脱离程度>3/4象限者视网膜下液的苯丙氨酸9.85±1.21mg/L、组氨酸20.63±2.07、血管内皮生长因子718.69±283.34μg/L高于<1/2象限者和1/2～3/4象限者，且差异具有统计学意义(P<0.05)。孔源性视网膜脱离患者视网膜下液VEGF与苯丙氨酸呈正相关(r=0.542，P<0.001)，与组氨酸呈正相关(r=0.782，P<0.001)。

结论：孔源性视网膜脱离患者的视网膜下液中氨基酸和VEGF的表达高于正常对照组，且随脱离程度的增加而上升。

METHODS: Forty-eight patients(52 eyes)with rhegmatogenous retinal detachment treated in our hospital were selected. According to the degree of retinal detachment, patients were divided into <1/2 quadrant group, 1/2-3/4 quadrant group and >3/4 quadrant group. Fifty-five healthy objects for physical examination in our hospital were selected as the control group,to compare the differences of amino acids and VEGF levels in the serum. Correlation analysis on VEGF levels and amino acids in the serum and in subretinal fluid among patients with different grades of rhegmatogenous retinal detachment was conducted.

RESULTS: In patients with rhegmatogenous retinal detachment, the tryptophan in serum was 28.59±4.46mg/L, phenylalanine 8.95±2.55mg/L, methionine 8.15±2.17mg/L, valine 28.62±5.29mg/L, histidine 18.96±1.85mg/L and VEGF 589.92±185.34μg/L, which were higher than those in the control group, and the difference was statistically significant(P<0.05). The levels of phenylalanine was 9.85±1.21mg/L, histidine 20.63±2.07mg/L and VEGF 718.69±283.34μg/L in the subretinal fluid of >3/4 quadrant group, which were significantly higher than those in the <1/2 quadrant group and 1/2-3/4 quadrant group(P<0.05). VEGF in the subretinal fluid of VEGF in the rhegmatogenous retinal detachment group were positively correlated with phenylalanine(r=0.542, P<0.001), and histidine(r=0.782, P<0.001).

CONCLUSION: The levels of amino acids and VEGF in the subretinal fluid of patients with rhegmatogenous retinal detachment was higher than those in normals and increased with the severity of retinal detachment.