方法：FS200型飞秒激光对眼库中期保存的供体角膜5只做深基质切削，将同一供体角膜分割为板层植片和内皮植片，预留后板层厚度大于150μm。激光频率200kHz，激光能量基质切削选择1.2μJ，边切能量1.2μJ。观察指标：角膜内皮瓣厚度、切割后的角膜组织学变化(HE染色)、电镜下角膜基质面规则程度评级。

结果：供体角膜平均厚度为534±24μm。预设角膜后板层厚度为199±7μm，获得后板层厚度在196±40μm(Z=-0.13，P=0.89)。角膜组织HE染色显示切削面略有不光滑。扫描电镜下，前板层基质面规则程度评分1.2±0.4分，角膜后板层基质规则程度评分为2.4±0.5分，差异有统计学意义(Z=-2.12，P=0.03)。

结论：FS200飞秒激光深基质切削精准性良好。FS200飞秒激光制备供体角膜的前板层较后板层基质面光滑。

METHODS: The FS200 laser cut 5 eye bank donors to get 5 anterior lamellar discs(ALD)and the corresponding 5 posterior lamellar discs(PLD). The thickness of PLD was not less than 150μm. The laser frequency was 200KHz. The lamellar cut energy was 1.2μJ and the trephination cut energy was 1.2μJ. The thickness of PLD, the histological changes of corneas(HE staining)and the degree of surface roughness under scanning electron microscope were recorded.

RESULTS: The corneal thickness before cutting was 534±24μm. The preset posterior lamellar corneal thickness was 199±7μm. The obtaining posterior lamellar thickness was 196±40 μm(Z=-0.13, P=0.89). With HE staining, the corneal cutting surface was slightly rough. Under the scanning electron microscopy, the smoothness score of the anterior lamellar stromal surface was 1.2±0.4, the posterior lamellar stromal surface smoothness score was 2.4±0.5(Z=-2.12, P=0.03).

CONCLUSION: The precision of deep corneal stroma cutting made by FS200 femtosecond laser is good. The cutting surface of the ALD is smoother than that of the PLD.