方法：研究患者玻璃体切除术中获取玻璃体样本55份。依据角膜表面曲率大小及眼轴长度，将研究对象分为两组：即高度近视组(25例)：SE>-6.00D， AL≥26.00mm和对照组或非高度近视组(30例)：SE≤-6.00D， AL<26.00mm。利用ELISA的方法测量研究对象玻璃体MMP-2和TGF-β2浓度。

结果：高度近视组玻璃体MMP-2浓度(96.87±55.95ng/mL)显著高于对照组(77.24±41.81ng/mL， P<0.05)，但与眼轴没有相关性(r=0.088， P=0.544)。高度近视组(3729.08±1890.88pg/mL)与对照组(3926.00±1333.88pg/mL， P<0.05)TGF-β2浓度具有显著差异性，且TGF-β2浓度与眼轴长度呈负相关(r=-0.344， P=0.014)。

结论：人玻璃体中MMP-2和TGF-β2两种分子浓度在高度近视进展中具有重要作用，TGF-β2浓度与眼轴长度具有相关性。

METHODS: The concentrations of MMP-2 and TGF-β2 levels were tested by Enzyme-Linked Immunosorbnent Assay(ELISA). Fifty-five human vitreous samples of 55 patients were collected during vitrectomy surgery, and were divided into two groups according to their spherical equivalent(SE)and axial length(AL). High myopia group(25 cases): SE>-6.00D, AL≥26.00mm, and control group or non-high myopia group(30 cases): SE≤-6.00D, AL<26.00mm.

RESULTS: The MMP-2 levels in vitreous of high myopia group(96.87±55.95ng/mL)was significantly higher than that of control group(77.24±41.81ng/mL, P<0.05), but not correlated with AL(r=0.088, P=0.544). While the TGF-β2 vitreous concentration was negatively correlated with AL(r=-0.344, P=0.014), and there was significant difference of TGF-β2 vitreous levels between high myopia group(3729.08±1890.88pg/mL)and control group(3926.00±1333.88pg/mL, P<0.05).

CONCLUSION: MMP-2 and TGF-β2 in human vitreous may play a critical role in human high myopia development, and the TGF-β2 appears to be associated with axial length.