[关键词]
[摘要]
目的：在体外实验中探讨雌激素对人角膜基质细胞中基质金属蛋白酶MMP-2及其抑制剂TIMP-2和转化生长因子TGF-β1蛋白表达的影响。
方法：用1.5ng/mL IL-1β模拟角膜基质细胞炎性环境，不同浓度雌激素(0，10-10，10-8，10-6，10-4mol/L的17-β雌二醇)体外作用于人角膜基质细胞，四甲基偶氮唑(MTT)比色法鉴定细胞活性。酶联免疫吸附测定(ELISA)法检测各组细胞上清中MMP-2、TIMP-2及TGF-β1蛋白的表达量。
结果：不同浓度雌激素(E2)对角膜基质细胞的活性没有影响。E2处理组的MMP-2、TIMP-2及TGF-β1表达量与对照组比较，差异均有统计学意义(P<0.05)。E2处理组与对照组相比，雌激素处理使MMP-2及TGF-β1的表达明显减少，而TIMP-2的表达则显著增多。
结论：雌激素能一定程度抑制MMP-2及TGF-β1的表达，同时促进TIMP-2的表达，这可能对正常人角膜起保护作用。
[Key word]
[Abstract]
AIM:To investigate the effects of estrogen on the expression of matrix metalloproteinases-2(MMP-2), tissue inhibitor of metalloproteinases-2(TIMP-2)and transforming growth factor- β1(TGF-β1)in cultured human corneal stromal cells.
METHODS: Inflammatory environments of human corneal stromal cells were simulated by using 1.5ng/mL IL-1β. The cells were then treated with or without different concentrations of estrogen(0, 1×10-4, 1×10-6, 1×10-8, 1×10-10mol/L estradiol)in vitro. Cell viability was evaluated by MTT. Expression levels of MMP-2, TIMP-2 and TGF-β1 proteins were measured by enzyme-linked immunosorbent assay(ELISA).
RESULTS:Estrogen did not affect the viability of human corneal stromal cells. Compared with the control group, expression levels of MMP-2 and TGF- β1 proteins in E2 treatment group significantly decreased after being treated with estrogen, while the expression level of TIMP-2 significantly increased.
CONCLUSION: Estrogen could, to some extent, down-regulate the expression of MMP-2 and TGF- β1 and up-regulate the expression of TIMP-2, which might contribute to protecting human cornea.
[中图分类号]
[基金项目]
国家自然科学基金资助项目(No.31200711)
