目的：检测原发性翼状胬肉患者泪液中肿瘤坏死因子α(tumor necrosis factor alpha，TNF-α)、γ干扰素(interferon gamma，IFN-γ)的含量，探讨翼状胬肉发生的泪液机制。

方法：用酶联免疫吸附方法(ELISA)对单眼原发性翼状胬肉患者60例60眼(静止期组30例30眼、活动期组30例30眼)和正常对照组健康志愿者30例30眼泪液中TNF-α、IFN-γ的含量进行定量测定，并进行统计学分析。

结果：原发性翼状胬肉患者患眼泪液中TNF-α、IFN-γ的含量与正常对照组比较，差异均具有统计学意义(P<0.05)。原发翼状胬肉患者患眼泪液中TNF-α含量均高于正常对照组，而IFN-γ含量均低于正常对照组，差异均有统计学意义(P<0.05)。

结论：泪液中TNF-α、IFN-γ含量的变化在促进翼状胬肉的发生发展中可能发挥重要作用，为原发性翼状胬肉的药物治疗提供了新思路。

METHODS: Concentrations of TNF-α and interferon gamma IFN-γ were quantified by enzyme-linked immunosorbent assay(ELISA)in asymptomatic control group(30 eyes of 30 healthy volunteers)and 60 primary pterygium patients(30 eyes of the 30 progressive cases, 30 eyes of the 30 quiescent cases). The data was analyzed by analysis of variance.

RESULTS: The concentration of TNF-α and IFN-γ in the patients with primary pterygium were statistically significant(P<0.05), compared with the normal control group. Tear concentration of TNF-α was significantly higher in primary pterygium compared with the control group. Tear concentration of IFN-γ was significantly reduced in primary pterygium compared with the control group(P<0.05).

CONCLUSION: TNF-α and IFN-γ may be important in the pathogenesis and development of pterygium tissues. It can provide some new directions and ideas for the treatment of primary pterygium.