Stereopsis is to perceive vision depth through the subtle three-dimensional differences between two eyes, and it is closely related to human working and living. There are different visual cortices in the cerebral cortex, which are involved in the formation of stereo vision, and process different visual information received. Clinical evaluation of stereo vision is needed to guide clinical decision-making. Traditional assessment is mainly based on static stereoscopic acuity, while binocular disparity appears dynamically with time in daily life. Nowadays 3D technology has been used to evaluate stereo vision. This paper mainly reviews the development mechanism and measurement methods of stereoscopic vision.