方法：收集2018-01/10在中国人民解放军第四七四医院行原发性翼状胬肉切除术的手术标本60例60眼(其中年龄<50岁30眼，年龄>50岁30眼)，同时间收集年龄匹配的斜视矫正术和视网膜脱离复位术患者的正常结膜组织30例30眼(其中年龄<50岁15眼，年龄>50岁15眼)。采用HE染色和免疫组织化学法检测SDF-1和CXCR4在翼状胬肉组织标本中的表达和定位，分析SDF-1和CXCR4的表达与患者临床表现的关系。使用IPP 6.0软件定量测定SDF-1和CXCR4的平均光密度。

结果：SDF-1和CXCR4在正常结膜上皮基底层细胞显示阳性或无阳性表达，而在翼状胬肉中全层结膜上皮细胞和血管内皮细胞均有阳性表达，其表达水平差异显著，基底层细胞中表达更明显，显示出明显极性。翼状胬肉组织中SDF-1和CXCR4的表达量均高于正常结膜组织(P<0.05)； 年龄<50岁患者的CXCR4表达量大于年龄>50岁患者的表达量(P<0.05)。

结论：SDF-1和CXCR4在翼状胬肉中表达上调，提示SDF-1和CXCR4参与翼状胬肉的形成，抑制SDF-1/CXCR4信号通路，可能抑制翼状胬肉发生，也可能成为翼状胬肉的药物治疗靶点，成为一个新的研究方向。年轻翼状胬肉患者CXCR4的表达更高，提示未来可能实现个体化给药，减少医疗资源的浪费。

METHODS: From January 2018 to October 2018, surgical specimens of 60 patients with primary pterygium and 60 eyes(including 30 eyes younger than 50 years old and 30 eyes older than 50 years old)were collected from the No.474 Hospital of Chinese PLA, at the same time collect age matched strabismus diorthosis and normal conjunctiva tissues of 30 patients with retinal detachment repairs(including 15 eyes younger than 50 years, older than 50 years of age 15 eyes).The expression and localization of SDF-1/CXCR4 in pterygium tissue specimens were detected by HE staining and immunohistochemistry, and the relationship between the expression of SDF-1/CXCR4 and the clinical characteristics of patients was analyzed. The mean optical density of SDF-1 /CXCR4 was measured by IPP 6.0 software.

RESULTS: SDF-1/CXCR4 showed slightly positive or no positive expression in normal conjunctival basal cells, but positive expression in both full-layer conjunctival epithelial cells and vascular endothelial cells in pterygium, with significant difference in expression level, and more obvious expression in basal cells, showing obvious polarity. The expression levels of SDF-1 and CXCR4 in pterygium tissues were higher than those in normal conjunctival tissues, and the difference was statistically significant(P<0.05). The expression of CXCR4 in patients younger than 50 years old was greater than that in patients older than 50 years old, and the difference was statistically significant(P<0.05).

CONCLUSION: SDF-1 and CXCR4 expression is up-regulated in pterygium, suggesting that SDF-1 and CXCR4 participates in the formation of pterygium and inhibits the SDF-1/CXCR4 signaling pathway, which may inhibit the occurrence of pterygium, and may also become a drug therapeutic target for pterygium, and become a new research direction. The higher expression of CXCR4 in young pterygium patients suggests that individualized drug administration may be realized in the future to reduce the waste of medical resources.